Investigating Viruses During the Transformation of Molecular Biology: Part II.
My scientific career started at an extraordinary time, shortly after the discoveries of the helical structure of DNA, the central dogma of DNA to RNA to protein, and the genetic code. Part I of this series emphasizes my education and early studies highlighted by the isolation and characterization of numerous vaccinia virus enzymes, determination of the cap structure of messenger RNA, and development of poxviruses as gene expression vectors for use as recombinant vaccines.
EHA105 Agrobacterium Strain |
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STR3004 | Lifescience Market | 100 ul | EUR 365 |
GV3101 Agrobacterium Strain |
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STR3005 | Lifescience Market | 100 ul | EUR 412 |
AGL1 Agrobacterium Strain |
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STR3006 | Lifescience Market | 100 ul | EUR 365 |
LBA4404 Agrobacterium Strain |
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STR3007 | Lifescience Market | 100 ul | EUR 365 |
GV2260 Agrobacterium Strain |
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STR3008 | Lifescience Market | 100 ul | EUR 339 |
EHA101 Agrobacterium Strain |
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STR3010 | Lifescience Market | 100 ul | EUR 365 |
C58 Agrobacterium Strain |
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STR3108 | Lifescience Market | 100 ul | EUR 329 |
GV3101 Electrocompetent Agrobacterium - 6x50µl |
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1282-12 | Intact Genomics | 6/PK | EUR 336 |
GV3101 Electrocompetent Agrobacterium - 18x50µl |
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1282-36 | Intact Genomics | 18/PK | EUR 669 |
AGL1 Electrocompetent Agrobacterium - 6x50µl |
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1283-12 | Intact Genomics | 6/PK | EUR 336 |
AGL1 Electrocompetent Agrobacterium - 18x50µl |
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1283-36 | Intact Genomics | 18/PK | EUR 669 |
EHA105 ElectroCompetent Agrobacterium- 6x50µl |
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1284-12 | Intact Genomics | 6/PK | EUR 336 |
EHA105 ElectroCompetent Agrobacterium- 18x50µl |
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1284-36 | Intact Genomics | 18/PK | EUR 669 |
LBA4404 ElectroCompetent Agrobacterium - 6x50µl |
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1285-12 | Intact Genomics | 6/PK | EUR 318 |
LBA4404 ElectroCompetent Agrobacterium- 18x50µl |
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1285-36 | Intact Genomics | 18/PK | EUR 669 |
A4 Agrobacterium tumefaciens Strains |
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S0068 | Lifescience Market | 100 ul | EUR 365 |
LBA9402 Agrobacterium tumefaciens Strains |
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S0069 | Lifescience Market | 100 ul | EUR 365 |
ATCC15834 Agrobacterium tumefaciens Strains |
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S0070 | Lifescience Market | 100 ul | EUR 365 |
EHA105 Agrobacterium tumefaciens Strains |
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S0071 | Lifescience Market | 100 ul | EUR 365 |
GV3101 Agrobacterium tumefaciens Strains |
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S0072 | Lifescience Market | 100 ul | EUR 412 |
AGL1 Agrobacterium tumefaciens Strains |
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S0073 | Lifescience Market | 100 ul | EUR 365 |
LBA4404 Agrobacterium tumefaciens Strains |
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S0074 | Lifescience Market | 100 ul | EUR 365 |
GV2260 Agrobacterium tumefaciens Strains |
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S0101 | Lifescience Market | 100 ul | EUR 365 |
EHA101 Agrobacterium tumefaciens Strains |
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S0104 | Lifescience Market | 100 ul | EUR 365 |
R1000 Agrobacterium tumefaciens Strains |
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S0107 | Lifescience Market | 100 ul | EUR 365 |
C58C1 Agrobacterium tumefaciens Strains |
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S0108 | Lifescience Market | 100 ul | EUR 365 |
P3301/ LBA4404 Agrobacterium Strain |
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STR3009 | Lifescience Market | 100 ul | EUR 720 |
p3301/ LBA4404 Agrobacterium tumefaciens Strains |
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S0102 | Lifescience Market | 100 ul | EUR 365 |
pSoup/ GV3101 Agrobacterium tumefaciens Strains |
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S0121 | Lifescience Market | 100 ul | EUR 365 |
pSoup/ EHA105 Agrobacterium tumefaciens Strains |
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S0126 | Lifescience Market | 100 ul | EUR 365 |
virD2 protein (Agrobacterium tumefaciens) (His tag) |
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80R-3437 | Fitzgerald | 50 ug | EUR 257 |
Description: Purified recombinant virD2 protein (Agrobacterium tumefaciens) (His tag) |
Agrobacterium sp.3-phosphoshikimate 1-carboxyvinyltransferase (aroA) |
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1-CSB-YP870830ALAE | Cusabio |
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Description: Recombinant Agrobacterium sp.3-phosphoshikimate 1-carboxyvinyltransferase(aroA) expressed in Yeast |
Agrobacterium sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) |
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1-CSB-EP870830ALAE | Cusabio |
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Description: Recombinant Agrobacterium sp. 3-phosphoshikimate 1-carboxyvinyltransferase(aroA) expressed in E.coli |
Agrobacterium sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) |
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1-CSB-MP870830ALAE | Cusabio |
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Description: Recombinant Agrobacterium sp. 3-phosphoshikimate 1-carboxyvinyltransferase(aroA) expressed in Mammalian cell |
Random Hexamers 100 µg |
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26-4000-03 | Gene Link | 100 ug | EUR 125 |
Description: Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3). References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5. |
AGS8830-8 PCR instrument |
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AGS8830 | Daan Gene | 1 unit/set | Ask for price |
RNA/DNA Purification Kit (Magnetic Bead) |
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DA0623 | Daan Gene | 32 test/kit | Ask for price |
Specimen Preservation Reagent |
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DA0970 | Daan Gene | 100 test/kit | Ask for price |
PCR detection kit for Monkeypox Virus |
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DA1430 | Daan Gene | 24 test/kit | EUR 108 |
Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970. |
Nucleic Acid Instrument |
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Smart-32 | Daan Gene | 1 unit/set | Ask for price |
Nucleic Acid Instrument |
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Swift-96 | Daan Gene | 1 unit/set | Ask for price |
Phospholipid transfer Antibody |
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20-abx110096 | Abbexa |
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Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2) |
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1-CSB-YP357288AYS | Cusabio |
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Description: Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein(virE2) expressed in Yeast |
Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2) |
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1-CSB-EP357288AYS | Cusabio |
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Description: Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein(virE2) expressed in E.coli |
Rat cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E02C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E02C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E02C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E03C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E03C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E03C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E01C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E01C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E01C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E07C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E07C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E07C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E06C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E06C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E06C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E04C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E04C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E04C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E08C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E08C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E08C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E09C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E09C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E09C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Phospholipid transfer Antibody (Biotin) |
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20-abx105774 | Abbexa |
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Phospholipid transfer Antibody (FITC) |
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20-abx107192 | Abbexa |
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Phospholipid transfer Antibody (HRP) |
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20-abx108612 | Abbexa |
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Phospholipid transfer Polyclonal Antibody |
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42297-100ul | SAB | 100ul | EUR 333 |
TRK Fused Gene (TRK fused gene) Antibody |
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abx239001-100ug | Abbexa | 100 ug | EUR 509 |
Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E05C0732-192T | BlueGene | 192 tests | EUR 1270 |
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E05C0732-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit |
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E05C0732-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit |
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GA-E1532HM-48T | GenAsia Biotech | 48T | EUR 289 |
Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit |
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GA-E1532HM-96T | GenAsia Biotech | 96T | EUR 466 |
Human cholest erolester transfer protein/lipid transfer protein,CETP/LTP ELISA Kit |
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201-12-1516 | SunredBio | 96 tests | EUR 440 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit |
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QY-E03917 | Qayee Biotechnology | 96T | EUR 361 |
Bovine Glycolipid transfer protein (GLTP) |
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1-CSB-YP009539BO | Cusabio |
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Description: Recombinant Bovine Glycolipid transfer protein(GLTP) expressed in Yeast |
Human Phospholipid transfer protein (PLTP) |
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1-CSB-YP018212HU | Cusabio |
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Description: Recombinant Human Phospholipid transfer protein(PLTP) expressed in Yeast |
Recombinant Glycolipid Transfer Protein (GLTP) |
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4-RPC502Hu01 | Cloud-Clone |
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Description: Recombinant Human Glycolipid Transfer Protein expressed in: E.coli |
Recombinant Phospholipid Transfer Protein (PLTP) |
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4-RPC728Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Phospholipid Transfer Protein expressed in: E.coli |
Recombinant Phospholipid Transfer Protein (PLTP) |
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4-RPC728Ra01 | Cloud-Clone |
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Description: Recombinant Rat Phospholipid Transfer Protein expressed in: E.coli |
Bovine Glycolipid transfer protein (GLTP) |
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1-CSB-EP009539BO | Cusabio |
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Description: Recombinant Bovine Glycolipid transfer protein(GLTP) expressed in E.coli |
Human Phospholipid transfer protein (PLTP) |
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1-CSB-EP018212HU | Cusabio |
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Description: Recombinant Human Phospholipid transfer protein(PLTP) expressed in E.coli |
SuperBlot Western Transfer buffer 10X |
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DG-WEST | Bio Basic | 500ml | EUR 113.8 |
Xpert Native Transfer Buffer(10X) |
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P9001-050 | GenDepot | 500ml | EUR 93 |
Xpert Native Transfer Buffer(10X) |
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P9001-100 | GenDepot | 1L | EUR 138 |
Glycolipid Transfer Protein (GLTP) Antibody |
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20-abx128414 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx131266 | Abbexa |
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Phospholipid Transfer Protein (PLTP) Antibody |
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20-abx131267 | Abbexa |
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Glycolipid Transfer Protein (GLTP) Antibody |
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20-abx112809 | Abbexa |
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Phosphatidylcholine Transfer Protein (PCTP) Antibody |
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20-abx114433 | Abbexa |
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Phosphatidylinositol Transfer Protein, alpha Antibody |
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20-abx114447 | Abbexa |
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Here I describe a shift in my research focus to combine molecular biology and genetics for a comprehensive understanding of poxvirus biology. The dominant paradigm during the early years was to select a function, isolate the responsible proteins, and locate the corresponding gene, whereas later the common paradigm was to select a gene, make a mutation, and determine the altered function. Motivations, behind-the-scenes insights, importance of new technologies, and the vital roles of trainees and coworkers are emphasized. Expected final online publication date for the Annual Review of Virology, Volume 7 is September 29, 2020. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.